Sage at AGBT, Marco Island

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Sage Science attends the Advances in Genome Biology and Technology (AGBT) conference in Marco Island FL,  Feb. 25-27 2010.

paired-end sequencing

NGS library construction

restriction fragment isolation

PCR band collection

sub-cloning

Each disposable cassette has 4 sample lanes and 1 lane for a DNA reference ladder (provided by Sage).  The sample lanes are physically separate, preventing any possibility of cross-contamination.  Size ranges to be extracted are entered in software, and a non-UV optical detection system tracks the DNA migration and correctly times the electro-elution “cut”.

run times:               30 – 90 min

loading vol:              40 ul

elution vol:               40 ul

loading  capacity:     up to20 ug of sheared genomic DNA

                               up to 200 ng of PCR product

min. cut range:         +/- 10% of average length of randomly sheared DNA

recovery efficiency:   >50%