Sage Science products have many uses in DNA sequencing, including:
- Library construction for paired-end or mate-pair sequencing
- Removing low molecular weight content for long-read sequencing
- Template preparation for clonal amplification of DNA on beads
- Library construction for chromatin immunoprecipitation (ChIP) techniques
- Preparing ultra HMW DNA libraries for long-range genomic applications
With Pippin Prep, BluePippin, or PippinHT, users can select their desired fragment length and the instrument will automatically select and collect those fragments, eliminating all others. With SageELF, users get 12 contiguous fractions from each sample, allowing them to construct libraries with multiple insert sizes for improved genome assembly accuracy while making the most of every sample. The SageHLS instrument can extract DNA fragments up to 2 Mb in size or purify large genes directly from cells.
For short-read sequencing, a cleanly-sized library improves sequence efficiency by providing fragments that are more ideal for the flow cell or substrate used, and the reagent chemistries that have been optimized for a given read length or performance. This often boosts data quality and efficiency by making structural variation and indel discovery more straightforward.
For long-read sequencing, it is usually beneficial to remove small fragments from the sample to best exploit the platform’s capabilities. The BluePippin and PippinHT feature a High-Pass feature which only collect fragments above a size cut-off threshold. The SageELF also handles larger DNA fragments, binning them into size fractions. The SageHLS specializes in ultra-HMW DNA and extracts directly from cell suspensions to prevent unwanted shearing.