Author Archives: Alex

SageELF Size Selection for PacBio Circular Consensus Sequencing

A new preprint has landed on BioRxiv that reports on high-accuracy circular consensus sequencing (CCS) on the PacBio Sequel. The study, “Highly-accurate long-read sequencing improves variant detection and assembly of a human genome”, is authored by PacBio and an impressive … more »

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Small-seq for single-cell small-RNA sequencing

September 2018 Authors: Michael Hagemann-Jensen, Ilgar Abdullayev, Rickard Sandberg and Omid R Faridani Info: A new protocol from researchers at the Karolinska Institutet and the Ludwig Institute for Cancer Research outline a method for making small RNA sequencing libraries from … more »

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Product Update: The High Pass Plus Cassette

The High Pass Plus™ gel cassette is the newest addition to the Pippin Family. As the name suggests, it is dedicated to our BluePippin “High-Pass” DNA size selection which has been a go-to method for increasing the read lengths for … more »

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Tracking Yellow Fever: New Study Aims To Tackle YFV Epidemics

Last year, we posted a story about an international team of scientists who embarked on a mission with Brazilian researchers to study the dangerous mosquito-borne Chikungunya virus (“Tracking Chikungunya: New Study Traces Outbreak Path”- we were pleased to donate a … more »

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Genomic and epidemiological monitoring of yellow fever virus transmission potential

August 2018 Authors: N.R. Faria, et al. Info: A large collaboration between international and Brazilian researchers used a suite of epidemiological, spatial, and genomic approaches to characterize Yellow Fever virus transmission in Brazil. The study potentially establishes a framework for … more »

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Highly sensitive detection of mutations in CHO cell recombinant DNA using multi‐parallel single molecule real‐time DNA sequencing

June 2018 Authors: Joseph Cartwright, Karin Anderson, Joseph Longworth, Philip Lobb, and David James Info: To improve the quantitative assessment of recombinant DNA in cell lines, University of Sheffield scientists and collaborators developed a PacBio-based method for high-throughput rDNA sequence … more »

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London Calling: Who Will Beat the 1 Mb Read?

Later this week, scientists will descend on Old Billingsgate, London, for Oxford Nanopore’s annual user event. Better known as London Calling, the meeting has become famous for talks full of cutting-edge nanopore sequencing results, novel protocols, and best practices. The … more »

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App Note: BluePippin Sizing Yields Longer MinION Reads

BluePippin users are generating longer reads from their Oxford Nanopore MinION sequencers, as demonstrated in a new app note from our colleagues at Nippon Genetics. The app note showcases a workflow used by Kazuharu Arakawa, an associate professor in the … more »

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geXc Boston: Check Out This Free Symposium on Computational Genomics

If you’re in the Boston area, don’t miss this Friday’s one-day symposium on genomics and computational biology at the Broad Institute. Organized by the Genomic Exchange Community, this geXc (pronounced “jexy”) event will bring together speakers from academia and industry … more »

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A Whole Genome Assembly of the Horn Fly, Haematobia irritans, and Prediction of Genes with Roles in Metabolism and Sex Determination

May 2018 Authors: Kranti Konganti, Felix Guerrero, Faye Schilkey, Peter Ngam, et al. Info: Researchers sequenced, assembled, and annotated the genome of horn fly, a blood-feeding insect that preys on cattle. The 1.14 Gb assembly shows the fly is most … more »

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