Author Archives: Alex

Highly sensitive detection of mutations in CHO cell recombinant DNA using multi‐parallel single molecule real‐time DNA sequencing

June 2018 Authors: Joseph Cartwright, Karin Anderson, Joseph Longworth, Philip Lobb, and David James Info: To improve the quantitative assessment of recombinant DNA in cell lines, University of Sheffield scientists and collaborators developed a PacBio-based method for high-throughput rDNA sequence … more »

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London Calling: Who Will Beat the 1 Mb Read?

Later this week, scientists will descend on Old Billingsgate, London, for Oxford Nanopore’s annual user event. Better known as London Calling, the meeting has become famous for talks full of cutting-edge nanopore sequencing results, novel protocols, and best practices. The … more »

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App Note: BluePippin Sizing Yields Longer MinION Reads

BluePippin users are generating longer reads from their Oxford Nanopore MinION sequencers, as demonstrated in a new app note from our colleagues at Nippon Genetics. The app note showcases a workflow used by Kazuharu Arakawa, an associate professor in the … more »

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geXc Boston: Check Out This Free Symposium on Computational Genomics

If you’re in the Boston area, don’t miss this Friday’s one-day symposium on genomics and computational biology at the Broad Institute. Organized by the Genomic Exchange Community, this geXc (pronounced “jexy”) event will bring together speakers from academia and industry … more »

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A Whole Genome Assembly of the Horn Fly, Haematobia irritans, and Prediction of Genes with Roles in Metabolism and Sex Determination

May 2018 Authors: Kranti Konganti, Felix Guerrero, Faye Schilkey, Peter Ngam, et al. Info: Researchers sequenced, assembled, and annotated the genome of horn fly, a blood-feeding insect that preys on cattle. The 1.14 Gb assembly shows the fly is most … more »

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Assembly and annotation of a draft genome sequence for Glycine latifolia, a perennial wild relative of soybean

April 2018 Authors: Qiong Liu, Sungyul Chang, Glen Hartman, and Leslie Domier Info: At the University of Illinois, scientists sequenced a wild perennial relative of the soybean plant, producing a 939 Mb draft assembly using linked reads from a single … more »

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Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

April 2018 Authors: Paola Gaiero, Hana Šimková, JanVrána, Federico Santiñaque, et al. Info: Scientists from Wageningen University & Research and other institutions embarked on a project to improve sample prep protocols for obtaining high molecular weight DNA from plant cells … more »

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ABRF: Tech Lovers Head to Myrtle Beach for Core Lab Meeting

Conference season is going strong. Next on our list: the annual meeting of the Association of Biomolecular Resource Facilities in Myrtle Beach, S.C. We never miss this conference — it’s a great event for tech-loving life science people like us. ABRF … more »

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Join Us at the Upcoming Long-Read Workshop at Jackson Lab

Long-read sequencing is steadily gaining traction as scientists realize its value for resolving regions of the genome that are intractable with short-read methods. From structural variants to repetitive or GC-rich regions, many clinically important stretches of the human genome just … more »

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Podcast: HMW DNA, Personalized Cancer Vaccines, and Structural Variation

There’s a great new podcast interview with Stanford clinical geneticist and oncologist Hanlee Ji about targeting extremely large stretches of DNA by combining CRISPR methods, automated DNA purification with the SageHLS instrument, and 10x Genomics technology. The approach works with … more »

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