Category Archives: Tips

Pippin Pulse

Posted on May 2, 2013 by Alex

Field Inversion Gel Electrophoresis       We’ve set up this post to provide Pippin Pulse users with a resource to keep updated on new protocols and to post references that might be useful. We also invite users to feel … more »

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Pippin Platform Recommended for Nextera Mate Pair Size Selection

Posted on January 24, 2013 by Alex

Illumina released new sample prep protocol guidelines for generating mate pair libraries with its Nextera kit, and we’re pleased to report that the Pippin platform is the recommended choice for automated size selection. You can check out the Nextera Mate … more »

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And Now, a Word from Our Customer Service Department

Posted on November 28, 2012 by Alex

Here at Sage Science, we are delighted to see more and more people signing up as customers of the Pippin platform. With so many instruments out in the wild, we thought it would be a good time to sit down … more »

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Band Capture with Midori Green on the BluePippin

Posted on September 10, 2012 by Alex

Our band capture cassettes, for use on the BluePippin, are designed to automatically collect a band or fragment of DNA from a sample and remove unwanted DNA and/or primer artifacts. We’ve provided users with some flexibility to manipulate the capture … more »

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BluePippin “Mid-Range” Size Selection: 18-27 kb

Posted on September 7, 2012 by Alex

We’re pleased to be shipping our Mid-Range size selection cassette (BMF7510) for the BluePippin. While our Low-Range cassette (BLF7510, 1kb-10kb) works for many mate-pair library construction protocols, we’ve been hearing from our customers that there is interest in creating larger … more »

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BluePippin:Collecting all DNA “Greater Than” 1kb

Posted on June 21, 2012 by Alex

We have received several requests to use the Pippin to collect all remaining DNA above a programmed base pair value from a sample. Although Pippins currently have the capability to collect all DNA after a run time threshold (using the … more »

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“Overflow Detection” definition on the Pippin Prep to be eliminated

Posted on April 17, 2012 by Alex

| | Pippin Prep users are likely familiar with our “overflow detection” cassette definitions that are found in the “Cassette Type” drop down menu of the Protocol Editor. We use these definitions because Pippin cassettes exhibit a phenomenon viagra cheap … more »

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ChIP-Seq sample prep tip – Interpreting the apparent presence of a secondary band in size-selected DNA

Posted on June 14, 2011 by Alex

ChIP-Seq analysis is used to study the control of gene expression by identifying DNA regions that are associated  with chromatin at the time of cellular analysis. The ChIP-Seq procedure typically involves cross-linking DNA to the associated chromatin proteins, sonication to … more »

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