Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis

February 2016

Authors:
Andrea D. Tyler, Sara Christianson, Natalie C. Knox, Philip Mabon, Joyce Wolfe, Gary Van Domselaar, Morag R. Graham, and Meenu K. Sharma

Info:
In this PLoS One publication, scientists in Manitoba compare the quality of libraries constructed with Illumina’s Nextera and TruSeq protocols for Mycobacterium tuberculosis, which has a particularly challenging genome. They determined that the TruSeq method yielded more uniform data quality and coverage. BluePippin was used for size selection prior to sequencing on a MiSeq.

Citation:

DOI: 10.1371/journal.pone.0148676

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