Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

February 2015

Authors:
Francine E. Garrett-Bakelman, Caroline K. Sheridan, Thadeous J. Kacmarczyk, Jennifer Ishii, Doron Betel, Alicia Alonso, Christopher E. Mason, Maria E. Figueroa, and Ari M. Melnick

Info:
In this publication in the Journal of Visualized Experiments, scientists at Weill Cornell Medical College and the University of Michigan describe a protocol to map DNA methylation patterns across the genome with next-gen sequencing instead of microarrays. Where input amounts allow, the approach calls for automated DNA size selection using Pippin Prep.

Citation:
J Vis Exp. 2015; (96): 52246.
DOI: 10.3791/52246

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