December 2017
Authors:
Ruben Boers, Joachim Boers, Bas de Hoon, Christel Kockx, et al.
Info:
Scientists at Erasmus Medical Center determined that analysis of genome-wide methylation is challenging when fragment sizes are too small and that better epigenetic results are possible with MeD-seq. The enzyme LpnPI, they found, “is blocked by a fragment size smaller than 32 bp.” The team used PippinHT to purify PCR products prior to sequencing on the Illumina HiSeq 2500.
Citation:
Genome Research
https://genome.cshlp.org/content/28/1/88