High depth, whole-genome sequencing of cholera isolates from Haiti and the Dominican Republic

September 2012

Authors:

Rachel Sealfon, Stephen Gire, Crystal Ellis, Stephen Calderwood, Firdausi Qadri, Lisa Hensley, Manolis Kellis, Edward T Ryan, Regina C LaRocque, Jason B Harris, Pardis C Sabeti

Info:
Study performs high-depth NGS on V. cholerae strains from Haiti and DR, some of which had been previously typed by less-thorough PacBio and Illumina studies. Bacterial DNA was fragmented by nebularization, size selected tight at 200bp on Pippin Prep, then prepared library (end-repair, add adapters, enrich PCR for 15 cycles) and sequenced on Illumina HiSeq.

Citation:
BMC Genomics 2012, 13:468

http://dx.doi.org/10.1186/1471-2164-13-468

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