Sage Science automated DNA sample prep tools are used in a wide range of applications. In each section below, we round up some of the great work our customers are doing.

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Next-Gen Sequencing

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Publication: Improved Protocols for Illumina Sequencing, Current Protocols in Human Genetics
Sage tool: Pippin Prep

Publication: Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution (video), JoVE
Sage tool: Pippin Prep

Customer blog: A Sage partnership, Broad Institute
Sage tool: Pippin Prep

Blog: TGAC Bioinformaticians Benefit from SageELF
Sage tools: BluePippin, SageELF

Blog: At Einstein Med School, ‘Extremely Reproducible’ Pippin Platforms Save Time and Money
Sage tools: Pippin Prep, PippinHT

Blog: 1,000th Pippin Customer: Cave Scientist Extraordinaire
Sage tool: Pippin Prep

Blog: For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies
Sage tools: BluePippin, Pippin Prep

Case Study: Tag Team: Scientists Use Pippin to Optimize Nextera Libraries, Zach Herbert, Dana-Farber Cancer Institute
Sage tool: Pippin Prep

Case Study: Better Metagenomics Methods Offer Hope for Studies with Ultra-Low DNA, Matthew Sullivan, University of Arizona
Sage tool: Pippin Prep


Long-Read Sequencing

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Publication: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations, BMC Genomics
Sage tools: Pippin Prep, BluePippin

Publication: Assembly and diploid architecture of an individual human genome via single-molecule technologies, Nature Methods
Sage tool: BluePippin

Poster: Taking Advantage of Long Read Lengths with Improved Library Preparation Methods
Sage tool: BluePippin

Poster: Single Molecule, Real-Time Sequencing of Full-length cDNA Transcripts Uncovers Novel Alternatively Spliced Isoforms
Tool: SageELF

Customer blog: Longing for the longest reads: PacBio and BluePippin, Norwegian Sequencing Centre
Sage tool: BluePippin

Customer blog: Increasing PacBio RS II SubRead Lengths, Institute for Genome Sciences
Sage tool: BluePippin

Case Study: Precise Sizing and SMRT Sequencing Offer Unprecedented Read Length for Clinical Studies, Robert Sebra, Icahn School of Medicine at Mount Sinai
Sage tool: BluePippin

App note: The effect of size selection of PacBio long-fragment libraries (≧7 kb), National Cancer Center Institute, Japan
Sage tools: BluePippin, Pippin Pulse

App note: Long-fragment library preparation for PacBio RS II – P5-C3 chemistry, Okinawa Institute of Advanced Sciences
Sage tool: BluePippin


MicroRNA / RNA-seq

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Blog: PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab
Sage tool: PippinHT

Blog: At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis
Sage tool: Pippin Prep

Blog: At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies
Sage tool: Pippin Prep

App note: Automated Size Selection of NEBNext Small RNA Libraries with the Sage Pippin Prep, New England Biolabs
Sage tool: Pippin Prep


Mate-Pair Sequencing

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Publication: A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost, Biotechniques
Sage tool: SageELF

Publication: An improved approach to mate-paired library preparation for Illumina sequencing
Sage tool: BluePippin

Publication: Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing, Biotechniques
Sage tool: BluePippin

Blog: TGAC Bioinformaticians Benefit from SageELF
Sage tools: BluePippin, SageELF

Blog: For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies
Sage tools: BluePippin, Pippin Prep

Blog: At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies
Sage tool: BluePippin

Blog: At Emory, Size Selection Saves Time for NGS Prep
Sage tool: Pippin Prep

App note: Size selection of long-chain fragments (8kb) in Nextera Mate Pair library preparation, University of Miyazaki, Japan
Sage tool: BluePippin


ChIP-seq

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Publication: Semiconductor-based DNA sequencing of histone modification states, Nature Communications
Sage tool: Pippin Prep

App note: ChIP-seq Library Prep, Dana-Farber Cancer Institute
Sage tool: Pippin Prep

Blog: PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab
Sage tool: PippinHT

Blog: At Boston University, Pippin Makes Quick Work of ChIP-seq Prep
Sage tool: Pippin Prep


ddRADseq / Massively Parallel Genotyping

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Blog: At NYU’s Center for Genomics and Systems Biology, Pippin Allows for More Precise DNA Size Selection
Sage tool: Pippin Prep

Case Study: New Take on RADseq Enables High-Throughput Variant Discovery, Brant Peterson, Harvard University
Sage tool: Pippin Prep


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