Citations

Highly sensitive detection of mutations in CHO cell recombinant DNA using multi‐parallel single molecule real‐time DNA sequencing

June 2018

Authors:
Joseph Cartwright, Karin Anderson, Joseph Longworth, Philip Lobb, and David James

Info:
To improve the quantitative assessment of recombinant DNA in cell lines, University of Sheffield scientists and collaborators developed a PacBio-based method for high-throughput rDNA sequence analysis. The protocol can sequence 40,000 individual recombinant DNA molecules at once to find point mutations. BluePippin was used for purification prior to sequencing.

Citation:
Biotechnology and Bioengineering
doi: 10.1002/bit.26561

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A Whole Genome Assembly of the Horn Fly, Haematobia irritans, and Prediction of Genes with Roles in Metabolism and Sex Determination

May 2018

Authors:
Kranti Konganti, Felix Guerrero, Faye Schilkey, Peter Ngam, et al.

Info:
Researchers sequenced, assembled, and annotated the genome of horn fly, a blood-feeding insect that preys on cattle. The 1.14 Gb assembly shows the fly is most closely related to Musca domestica and Drosophila melanogaster. BluePippin was used to size-select mate-pair libraries for genome and transcriptome sequencing using Illumina.

Citation:
G3: Genes, Genomes, Genetics
vol. 8 no. 5 1675-1686

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Assembly and annotation of a draft genome sequence for Glycine latifolia, a perennial wild relative of soybean

April 2018

Authors:
Qiong Liu, Sungyul Chang, Glen Hartman, and Leslie Domier

Info:
At the University of Illinois, scientists sequenced a wild perennial relative of the soybean plant, producing a 939 Mb draft assembly using linked reads from a single library on the 10x Genomics Chromium system. PippinHT was used to target the longest DNA fragments for analysis.

Citation:
The Plant Journal
doi: 10.1111/tpj.13931

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Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

April 2018

Authors:
Paola Gaiero, Hana Šimková, JanVrána, Federico Santiñaque, et al.

Info:
Scientists from Wageningen University & Research and other institutions embarked on a project to improve sample prep protocols for obtaining high molecular weight DNA from plant cells for optical mapping workflows. They cite BluePippin for use in a pulsed field gel electrophoresis step and use Pippin Pulse as well.

Citation:
MethodsX
Volume 5, 2018, Pages 328-336

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A Validation Approach of an End-to-End Whole Genome Sequencing Workflow for Source Tracking of Listeria monocytogenes and Salmonella enterica

March 2018

Authors:
Anne-Catherine Portmann, Coralie Fournier, Johan Gimonet, Catherine Ngom-Bru, et al.

Info:
Scientists from Nestle Research Center and Nestle Institute of Health Sciences aimed to establish guidelines for whole-genome sequencing used in contamination investigations in the food industry. The team used BluePippin for size selection prior to sequencing with PacBio.

Citation:
Front. Microbiol., 14 March 2018
doi: 10.3389/fmicb.2018.00446

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