Highly sensitive detection of mutations in CHO cell recombinant DNA using multi‐parallel single molecule real‐time DNA sequencing
Joseph Cartwright, Karin Anderson, Joseph Longworth, Philip Lobb, and David James
To improve the quantitative assessment of recombinant DNA in cell lines, University of Sheffield scientists and collaborators developed a PacBio-based method for high-throughput rDNA sequence analysis. The protocol can sequence 40,000 individual recombinant DNA molecules at once to find point mutations. BluePippin was used for purification prior to sequencing.
Biotechnology and Bioengineering
A Whole Genome Assembly of the Horn Fly, Haematobia irritans, and Prediction of Genes with Roles in Metabolism and Sex Determination
Kranti Konganti, Felix Guerrero, Faye Schilkey, Peter Ngam, et al.
Researchers sequenced, assembled, and annotated the genome of horn fly, a blood-feeding insect that preys on cattle. The 1.14 Gb assembly shows the fly is most closely related to Musca domestica and Drosophila melanogaster. BluePippin was used to size-select mate-pair libraries for genome and transcriptome sequencing using Illumina.
G3: Genes, Genomes, Genetics
vol. 8 no. 5 1675-1686
Assembly and annotation of a draft genome sequence for Glycine latifolia, a perennial wild relative of soybean
Qiong Liu, Sungyul Chang, Glen Hartman, and Leslie Domier
At the University of Illinois, scientists sequenced a wild perennial relative of the soybean plant, producing a 939 Mb draft assembly using linked reads from a single library on the 10x Genomics Chromium system. PippinHT was used to target the longest DNA fragments for analysis.
The Plant Journal
Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species
Paola Gaiero, Hana Šimková, JanVrána, Federico Santiñaque, et al.
Scientists from Wageningen University & Research and other institutions embarked on a project to improve sample prep protocols for obtaining high molecular weight DNA from plant cells for optical mapping workflows. They cite BluePippin for use in a pulsed field gel electrophoresis step and use Pippin Pulse as well.
Volume 5, 2018, Pages 328-336
A Validation Approach of an End-to-End Whole Genome Sequencing Workflow for Source Tracking of Listeria monocytogenes and Salmonella enterica
Anne-Catherine Portmann, Coralie Fournier, Johan Gimonet, Catherine Ngom-Bru, et al.
Scientists from Nestle Research Center and Nestle Institute of Health Sciences aimed to establish guidelines for whole-genome sequencing used in contamination investigations in the food industry. The team used BluePippin for size selection prior to sequencing with PacBio.
Front. Microbiol., 14 March 2018