Comparative Analysis of Ralstonia solanacearum Methylomes
April 2017
Authors:
Ivan Erill, Marina Puigvert, Ludovic Legrand, Rodrigo Guarischi-Sousa, et al.
Info:
Scientists report results of analyzing epigenetic patterns in two strains of a plant pathogen. They determined that methylation regulates virulence and adaptation to different host plants. BluePippin was used to remove smaller library fragments prior to sequencing with PacBio.
Citation:
Front Plant Sci. 2017; 8: 504
doi: 10.3389/fpls.2017.00504
Tripartite species interaction: eukaryotic hosts suffer more from phage susceptible than from phage resistant bacteria
April 2017
Authors:
Carolin Wendling, Agnes Piecyk, Dominik Refardt, Cynthia Chibani, et al.
Info:
Scientists investigated the extent to which a bacterium’s susceptibility or resistance to prophages alters its own effect as a pathogen, finding that bacteria that were more likely to take in phages were also more harmful to their eukaryotic host organism. Whole genome sequencing was performed with PacBio, and BluePippin was used to prepare 10 Kb libraries.
Citation:
BMC Evolutionary Biology 2017 17:98
The RNA Exosome Syncs IAV-RNAPII Transcription to Promote Viral Ribogenesis and Infectivity
April 2017
Authors:
Alexander Rialdi, Judd Hultquist, David Jimenez-Morales, Zuleyma Peralta, et al.
Info:
Scientists in New York and California teamed up to study the role of nuclear RNA exosomes in viral transcription, using influenza A. They found that the exosome coordinates transcription and that viral growth is suppressed when the RNA exosome’s activity is reduced. In the Illumina sequencing workflow, BluePippin was used to size-select PCR products.
Citation:
Cell
Impaired human immunodeficiency virus type 1 replicative fitness in atypical viremic non-progressor individuals
March 2017
Authors:
Jan Weber, Richard Gibson, Lenka Sácká, Dmytro Strunin, et al.
Info:
In this publication, scientists describe an analysis of the rates of HIV progression in several patients. They determined that reduced viral replicative fitness appears to be associated with patients whose infections do not progress to advanced AIDS. Deep sequencing of the HIV-1 genome was performed on the Ion PGM after size-selection with Pippin Prep.
Citation:
AIDS Res Ther (2017) 14:15
DOI 10.1186/s12981-017-0144-0
Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
March 2017
Authors:
Warren Cheung, Xiaojian Shao, Andréanne Morin, Valérie Siroux, et al.
Info:
Researchers at McGill University and collaborating institutions used ChIP-seq to elucidate the effects of epigenetics and phenotype across the human genome. They analyzed more than 900 samples to understand the effects of epigenetic regulation. The team used Pippin Prep to size-select fragments prior to sequencing on an Illumina HiSeq 2000.
Citation:
Genome Biology (2017) 18:50
DOI 10.1186/s13059-017-1173-7