Citations

H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis

March/April 2013

Authors:
Kyle C. Costa, Thomas J. Lie, Michael A. Jacobs, John A. Leigh

Info:
The authors constructed deletions in several key metabolism genes to study the mechanism of H2 independent growth in this hydrogenotropic strain. Several mutants were found that shed new light on alternative mechanisms of methanogenic growth. The mutants were mapped by whole genome sequencing, in which Pippin Prep was used to size select the libraries.

Citation:
mBio (4)2:00062-13 March/April 2013

http://dx.doi.org/10.1128/mBio.00062-13

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Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential

January/February 2013

Authors:
Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana

Info:
Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome sequence of one such strain. Nextera chemistry was used with size selection on the Pippin Prep to generate the sequencing libraries.

Citation:
genomeA (1)1:e00144-12 January/February 2013

http://dx.doi.org/10.1128/genomeA.00144-12

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A genome-wide survey of genetic variation in gorillas using reduced representation sequencing

January 2013

Authors:
Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith

Info:
The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion followed by size selection on the Pippin Prep for AluI fragments between 150-250 bp.

Citation:
pre-print

arXiv:1301.1729

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Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene

January 2013

Authors:
Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins

Info:
The authors designed a general method to study the arrangement of foreign DNA and host DNA rearrangements in trangenic animals. The protocol involves standard Illumina PE library generation, using the Pippin Prep for size selection, followed by microarray hybridization capture to isolate fragments containing the transgene.

Citation:
Nucleic Acids Research, 2013, 1-5

doi:10.1093/nar/gks1463

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Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice

January 2013

Authors:
Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra

Info:
The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species of wild mice with distinct burrowing behavior. Pippin Prep was used for size selection in their RADseq protocol.

Citation:
Letter, Nature 493:402-405

doi:10.1038/nature11816

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