Citations

Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice

January 2013

Authors:
Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra

Info:
The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species of wild mice with distinct burrowing behavior. Pippin Prep was used for size selection in their RADseq protocol.

Citation:
Letter, Nature 493:402-405

doi:10.1038/nature11816

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A Hybrid Genetic Linkage Map of Two Ecologically and Morphologically Divergent Midas Cichlid Fishes(Amphilophus spp.) Obtained by Massively Parallel DNA Sequencing (ddRADSeq)

January 2013

Authors:
Hans Recknagel, Kathryn R. Elmer, and Axel Meyer

Info:
RADseq was used to develop linkage maps for two highly divergent Cichlid fish. Pippin Prep was used to size select the libraries.

Citation:
G3 (Bethesda) 3(1):65-74 January 2013

http://dx.doi.org/10.1534/g3.112.003897

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IL-10 Produced by Induced Regulatory T Cells (iTregs) Controls Colitis and Pathogenic Ex-iTregs during Immunotherapy

December 2012

Authors:
Erica G. Schmitt, Dipica Haribhai, Jason B. Williams, Praful Aggarwal, Shuang Jia, Louis-Marie Charbonnier, Ke Yan, Rachel Lorier, et al.

Info:
The study included sequencing of the T-cell receptor. For this sequencing, the authors used Ion Torrent platform. The Pippin Prep was used for size-selection as per the standard Life/Ion library construction protocols.

Citation:
J Immunology 189(12): 5638-5648: December 2012

http://dx.doi.org/10.4049/​jimmunol.1200936

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Bactobolin Resistance is Conferred by Mutations in the L2 Ribosomal Protein

December 2012

Authors:
Josephine Chandler, Thao T. Truong, Patricia M. Silva, Mohammad R. Seyedsayamdost, Galvin Carr, Matthew Radey, Michael A. Jacobs, Elizabeth H. Sims, Jon Clardy, E. Peter Greenberg

Info:
Authors were searching for cause of resistance to antibiotic Bactobolin. Performed Illumina paired-end sequencing on resistant strains. Library construction used size-selection of library on Pippn Prep. Discovery of mutation was straightforward for two of four strains. Other two strains harbored a three base insertion which was not recognized in the initial Illumina sequencing.

Citation:
mBio 3(6):e00499-12: December 2012

http://dx.doi.org/10.1128/​mBio.00499-12

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SATB1-mediated functional packaging of chromatin into loops

November 2012

Authors:
Terumi Kohwi-Shigematsu, Yoshinori Kohwi, Keiko Takahashi, Hunter W. Richards, Stephen D. Ayers, Hye-Jung Han, Shutao Cai

Info:
Among other things, this paper outlines methods for mapping all DNA binding sites for a global chromatin organizing protein, SATB1. Recombinant HIS-tagged SATB1 protein is incubated with sheared genomic DNA, SATB1 with bound DNA is captured on magnetic HIS particles, and then DNA libraries are prepared from the particle-bound DNA. Illumina library construction is used, with size selection on the Pippin Prep (150-500bp).

Citation:
Methods: November 2012

http://dx.doi.org/10.1016/j.ymeth.2012.06.019,

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