Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression
Linzhao Cheng, Nancy F. Hansen, Ling Zhao, Yutao Du, Chunlin Zou, Frank X. Donovan, Bin-Kuan Chou, Guangyu Zhou, Shijie Li, Sarah N. Dowey, Zhaohui Ye, NISC Comparative Sequencing Program,
Settara C. Chandrasekharappa, Huanming Yang, James C. Mullikin, and P. Paul Liu
Whole genome sequencing on reprogrammed stem cell lines. Materials and Methods: Covaris shearing followed by size selection at 450 bp on the Pippin Prep. Illumina TruSeq chemistry on HiSeq 2000, 90 or 101 bp paired end protocols.
Cell Stem Cell 10, 337–344, March 2, 2012
Heather Fairfield, Griffith J Gilbert, Mary Barter, Rebecca R Corrigan, Michelle Curtain, Yueming Ding, Mark D’Ascenzo, Daniel J Gerhardt, Chao He, Wenhui Huang, Todd Richmond, Lucy Rowe, Frank J Probst,
David E Bergstrom, Stephen A Murray, Carol Bult, Joel Richardson, Benjamin T Kilew, Ivo Gut, Jorg Hager, Snaevar Sigurdsson, Evan Mauceli, Federica Di Palma, Kerstin Lindblad-Toh, Michael L Cunningham,
Timothy C Cox, Monica J Justice, Mona S Spector, Scott W Lowe, Thomas Albert, Leah Rae Donahue, Jeffrey Jeddeloh, Jay Shendure and Laura G Reinholdt
Materials and Methods: Illumina PE libraries were constructed and size selected at 430bp “tight” setting on Pippin Prep prior to enrichment PCR and hybridization capture for whole exome regions.
Genome Biology 2011, 12:R86
Laura G. Reinholdt, Yueming Ding, Griffith T. Gilbert, Anne Czechanski, Jeffrey P. Solzak, Randall J. Roper, Mark T. Johnson, Leah Rae Donahue, Cathleen Lutz, Muriel T. Davisson
Illumina PE libraries were constructed and size selected at 400bp “tight” setting on Pippin Prep prior to enrichment PCR and hybridization capture for genomic region surrounding the Down syndrome locus in mouse, to identify rearrangements in a much-studied Down’s syndrome model strain.
Mamm Genome (2011) 22:685–691