MENLO PARK, Calif. and BEVERLY, Mass. — May 16, 2013 — Pacific Biosciences of California, Inc., (NASDAQ:PACB), provider of the PacBio® RS II DNA Sequencing System, and Sage Science, a developer of life science products for improving sample preparation processes in molecular biology applications, today announced a co-marketing partnership to provide customers of the PacBio RS II the ability to sequence even longer DNA fragments when performing Single Molecule, Real-Time (SMRT®) Sequencing.
“We have found that the BluePippin™ size selection platform is very useful for applications where long read lengths are important, such as de novo genome assembly,” said Kevin Corcoran, Senior Vice President of Market Development at Pacific Biosciences. “Our studies have demonstrated that size selection with Sage’s BluePippin platform is an easy addition to our customers’ sample prep workflow that selects for 10 kb or larger fragments for sequencing on the PacBio RS II.”
Based on PacBio’s revolutionary SMRTSequencing technology, the PacBio RS II can generate average read lengths of 5,000 base pairs, with the longest reads above 20,000 base pairs in length. The PacBio RS II System, including consumables and software, provides a simple, fast, end-to-end workflow for SMRT Sequencing in research areas including infectious disease and microbiology, agriculture, and complex genetic diseases with repeat expansions.
The BluePippin platform, part of the Pippin line of automated size-selection tools offered by Sage Science, uses pulsed-field power to take fractions of DNA from fragments ranging from 50 bases to 50 kilobases. Independent studies of size-selection methods for next-generation sequencing sample prep have repeatedly found that the Pippin platform offers unparalleled reproducibility, accuracy, and sample recovery. By eliminating low-molecular-weight templates from sequencing libraries, automated size selection allows users to load longer fragments and boost the average read length produced by sequencing.
“This is excellent validation of the tremendous utility BluePippin offers for libraries designed to generate extraordinarily long sequence reads,” said Alex Vira, Director of Marketing for Sage Science. “We look forward to working with PacBio and its customers as they continue to deliver industry-leading read lengths and conduct countless studies that would be impossible with shorter-read technologies.”
In data presented at the Advances in Genome Biology & Technology conference earlier this year, Pacific Biosciences scientists assessed three libraries (bacterial, fish and mammalian) generated with and without BluePippin size selection. They reported that N50 subread lengths increased by 90%, 110%, and 86%, respectively, in the libraries that were size selected with BluePippin compared to those that were not.
About Pacific Biosciences
Pacific Biosciences of California, Inc. (Nasdaq:PACB) offers the PacBio® RS II DNA Sequencing System to help scientists solve genetically complex problems. Based on its novel Single Molecule, Real-Time (SMRT®) technology, the company’s products enable: targeted sequencing to more comprehensively characterize genetic variations; de novo genome assembly to more fully identify, annotate and decipher genomic structures; and DNA base modification identification to help characterize epigenetic regulation and DNA damage. By providing access to information that was previously inaccessible, Pacific Biosciences enables scientists to increase their understanding of biological systems. More information is available at www.pacb.com.
BEVERLY, Mass. — January 14, 2013 — Sage Science, a developer of life science products for improving sample preparation processes in molecular biology applications, today announced the launch of Pippin Link and Pippin Pulse, two new products designed to streamline library prep workflow and enhance the utility of the Pippin line of automated DNA size selection products.
The Pippin Link is an accessory for the Pippin Prep and BluePippin that lets users boost throughput by connecting up to eight instruments and controlling them from a single computer. This streamlines workflow and conserves bench space. The Pippin Pulse is a pulsed-field electrophoresis power supply for analytical gels. Based on the power supply of the BluePippin, Pippin Pulse is an affordable tool that lets researchers resolve DNA to 100 Kb and beyond. It uses a simple software interface that provides users full flexibility in developing their own field-inversion protocols.
“The trend we have seen is that scientists are buying multiple Pippin size selection instruments for their labs, so we developed the Pippin Link specifically for those customers to keep the process as simple and hands-off as possible,” said Chris Boles, Chief Scientific Officer of Sage Science. “Our other new release, Pippin Pulse, was designed for researchers who are trying to resolve very large DNA fragments and are looking for a low-cost, straightforward solution.”
Independent studies of size selection methods have repeatedly demonstrated that the Pippin platform offers unparalleled reproducibility, accuracy, and sample recovery. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing project costs.
“With the launch of these products, we’re pleased to be adding even more flexibility and multiplexing options for our customers,” said Alex Vira, Director of Marketing for Sage Science. “Precise size selection is a fundamental element for a successful sequencing project, and these new tools will improve researchers’ ability to get the best results with a more efficient workflow.”
BEVERLY, Mass. February 1, 2012 — Sage Science, Inc. today announced the introduction of the BluePippin, an instrument that automates the size selection and retrieval of DNA for molecular biology research. The BluePippin expands Sage’s Pippin line of preparative electrophoresis products by providing the capability to collect larger, kilobase-sized, DNA fragments. The Pippin platform is typically used to construct genomic libraries for next-gen sequencing.
The BluePippin is an updated version of Sage’s Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal hands-on time. The system will also feature a new optical design that improves the automated capture of DNA bands and restriction fragments.
Sage Science’s CEO, Gary Paul Magnant, commented on the BluePippin. “ We are pleased to be providing researchers an even more flexible alternative to a difficult manual procedure. The BluePippin pulsed-field feature should help enable next-gen sequencing techniques such as mate-pair sequencing, and address third generation sequencing where the requirement of intact high molecular weight DNA is critical. We also expect that our upper range of size selection will continue to increase with development, which should address an ever broader range of laboratory applications.”
Sage Science Pippin Prep To Be Commercialized Through Life Technologies For Ion PGM™ Sequencer Customers
Beverly, MA—October 11, 2011—Sage Science, Inc., a privately-held, MA-based company, today announced that it has signed an agreement with Life Technologies Corporation (NASDAQ:LIFE) enabling Life Technologies to offer the Pippin Prep DNA size selection system to users of the Ion Torrent PGM next-gen sequencing platform.
The Pippin Prep is an automated gel electrophoresis system that is designed for preparation of size-fractionated DNA samples. Automated workflow with the Pippin Prep improves speed, yield, quality,and reproducibility of next-gen library construction.
Sage CEO Gary Paul Magnant commented on the agreement: “Sage Science is thrilled to be working with Ion Torrent users and Life Technologies. We believe the Pippin Prep sets the standard for tightly sized libraries of the highest quality, and we look forward to supporting the emerging desktop sequencer market.”
Agilent Technologies and Sage Science Sign Agreement to Comarket Bioanalyzer and Pippin Prep DNA Systems
WALDBRONN, Germany, and BEVERLY, Mass., Feb. 1, 2011 — Agilent Technologies Inc. (NYSE: A) and Sage Science Inc. today announced that they have signed a comarketing agreement involving Agilent’s 2100 Bioanalyzer, used for the detection and quantification of nucleic acids, and Sage’s Pippin Prep DNA size-selection system.
The agreement between Sage and Agilent includes the copromotion of the respective products and development of application studies that will leverage the synergies of the two systems for next-generation sequencing applications.
“We are very pleased to be working with Agilent; our companies’ products are clearly complementary for the size-selection steps in next-generation sequencing,” said Gary Magnant, Sage CEO. “The high sensitivity of the 2100 Bioanalyzer kits provides superb analysis of DNA size distributions, with minimal sample consumption.”
Proper preparation and quality control of the input sample, as well as the library, are essential to achieving high-fidelity data in the next-generation sequencing workflow. The Pippin Prep, which automates DNA size selection for creating libraries, together with Agilent’s 2100 Bioanalyzer, the market-leading tool for sample and library quality control, advances and streamlines sample preparation.
“The Pippin Prep instrument from Sage and Agilent’s Bioanalyzer together offer a synergistic set of tools that enable researchers working with next-generation sequencing platforms to advance their research faster and more efficiently,” said Knut Wintergerst, electrophoresis marketing and support manager, Agilent.