The Pippin DNA size selection system consists of an electrophoresis instrument and pre-cast, disposable, gel cassettes. Using a proprietary branched-channel configuration with switching electrodes, DNA fractions are electro-eluted from agarose, and collected in buffer. DNA collection ranges are entered into software and automatically extracted.For collections of smaller fragments up to 1.5 kb, the Pippin Prep facilitates the construction of next-gen sequencing libraries for paired-end sequencing, ChIP seq, and preparing templates for emulsion PCR. If the collection of larger fractions is also needed, the BluePippin expands the upper size selection to fragments up to 50 kb using pulsed-field electrophoresis.
Key Benefits of Automated Preparative Electrophoresis
With just 1-2 minutes of effort per sample, there is a huge improvement over manual prep gels. Samples are collected in buffer, and removed with a standard pipette.
Quality and Consistency
This chart shows a comparison of manual gel libraries vs Pippin libraries for a paired-end sequencing pipeline over a two year period. After switching to the Pippin, a far more reproducible library was provided. In addition, there is no sample cross-contamination and efficient exclusion of adapter-dimers.