High Throughput Size Selection for Next-Gen Sequencing
Size Select Up To 24 Samples In One Run
PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.
Benefits of the PippinHT System
- Sample-to-sample reproducibility streamlines research
- Higher sample quality improves downstream analysis
- 24 sample capacity meets high throughput sequencing demands
Key Downstream Technologies
- whole genome sequencing
- miRNA library isolation
Pre-Cast Agarose Gel Cassettes for DNA Size Selection
Gel cassettes kit are available in several configurations to provide a full range of options. Size select DNA in ranges between 100bp up to 1.5kb. Also, High-Pass protocols are available for collecting all larger DNA above a fragment size.
Gel Cassette Types and Target Ranges
- 3% agarose, 100-250bp
- 2% agarose, 100-600bp
- 1.5% agarose, 300bp-1.5kb
- 0.75% agarose, high-pass size selection
- Paired-end sequencing
- miRNA isolation
- DNA sequencing, RNA-seq, genotyping
- DNA sequencing, ChIP-seq
See the Workflow Summary.
The PippinHT instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.
|Electrophoresis voltage||75V constant, 75V pulsed field|
|Optical detection||470 nm excitation, 525 nm emission|
|Power requirements||100-240 VAC, 2.5 A, 50-60 Hz|
|Weight||20 lbs / 9 kg|
|Dimensions (with lid open)||18h x 13w x 17d (in) | 46h x 33w x 64 d (cm)|
Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.