PippinHT

High Throughput Size Selection for Next-Gen Sequencing

Size Select Up To 24 Samples In One Run

PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin.  The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.

Benefits of the PippinHT System

  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis
  • 24 sample capacity meets high throughput sequencing demands

Key Downstream Technologies

  • whole genome sequencing
  • miRNA library isolation

Pre-Cast Agarose Gel Cassettes for DNA Size Selection

Gel cassettes kit are available in several configurations to provide a full range of options.   Size select DNA in ranges between 100bp up to 1.5kb.  Also, High-Pass protocols are available for collecting all larger DNA above a fragment size.

Gel Cassette Types and Target Ranges

  • 3% agarose, 100-250bp
  • 2% agarose, 100-600bp
  • 1.5% agarose, 300bp-1.5kb
  • 0.75% agarose, high-pass size selection

Downstream Technologies

  • Paired-end sequencing
  • miRNA isolation
  • DNA sequencing, RNA-seq, genotyping
  • DNA sequencing, ChIP-seq

See the Workflow Summary.

Instrument

The PippinHT instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.

PippinHT Specs
Electrophoresis voltage75V constant, 75V pulsed field
Optical detection470 nm excitation, 525 nm emission
Power requirements100-240 VAC, 2.5 A, 50-60 Hz
Weight20 lbs / 9 kg
Dimensions (with lid open)18h x 13w x 17d (in) | 46h x 33w x 64 d (cm)

Cassette Kits

Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.

PippinHT Citations

PippinHT Blog Posts