SageELF

Whole Sample Fractionation of DNA or Protein

Collect 12 Contiguous Fractions from a Single Sample

The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions.  The system is equipped with pulsed-field electrophoresis for resolving large DNA.

Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer. One sample is fractionated on a single precast agarose cassette, and one or two cassettes may be processed at one time.

Benefits of the SageELF System:                                       

  • Reduces the complexity of RNA (cDNA) or protein samples
  • Allows construction of DNA libraries with multiple insert sizes for improved assembly
  • Allows sequencing of valuable DNA and retrieving sample for later study

Key Downstream Technologies:

  • Proteomics and mass spec
  • Mate-pair sequencing
  • Splice variant detection

Pre-Cast Agarose Gel Cassettes for DNA Fractionation

Gel cassettes are available in two agarose gel concentrations to provide whole-sample DNA fractionation in ranges between 100 bp and 40 kb.

Gel cassette types and
target ranges

  • 2% agarose, 100 bp – 2.3 kb
  • 0.75% agarose,   1 kb – 18 kb
  • 0.75% agarose, 10 kb –40 kb

For these downstream
technologies

  • miRNA and RNA-seq
  • DNA sequencing, RNA-seq
  • Mate-pair sequencing

Pre-Cast SDS-Agarose Gel Cassettes for Protein Fractionation

Gel cassettes for protein fractionation are available in two agarose concentrations to provide a full range of options for proteomic studies.

Gel cassette types
and target ranges

  • 5% agarose, 10 – 100 kDa
  • 3% agarose, 50 – 500 kDa

For these downstream
technologies

  • Protein mass spec
  • Mass spec and protein complexes

Instrument:

The SageELF instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.

Sage Elf Specs
Electrophoresis voltage:25V to 150V constant, adjustable, or pulsed field
Optical detector:470 nm excitation | 525 nm emission
Power requirements:100-240 VAC, 2.5 A, 50-60 Hz
Weight:20 lbs / 9 kg
Dimensions10.5h x 13.5w x 10.5d (in) | 27h x 34w x 27 d (cm)

Cassette Kits             

Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.

 

SageELF Citations

SageELF Blog Posts