Tag Archives: BluePippin

Comparison of mitochondrial DNA variants detection using short- and long-read sequencing

August 2019 Authors: Ahmed N. Alkanaq, Kohei Hamanaka, Futoshi Sekiguchi, Masataka Taguri, Atsushi Takata, Noriko Miyake, Satoko Miyatake, Takeshi Mizuguchi, Naomichi Matsumot Info: The genotypes of mitochondrial DNA variants are compared using both short-read (Illumina Hi-SeqX10) and long-read (PacBio Sequel) … more »

Posted in Citation | Tagged , , | Comments Off on Comparison of mitochondrial DNA variants detection using short- and long-read sequencing

Structural variants identified by Oxford Nanopore PromethION sequencing of the human genome

June 2019 Authors: De Coster Wouter, De Rijk Peter, De Roeck Arne, De Pooter Tim, D’Hert Svenn, Strazisar Mojca, Kristel Sleegers and Van Broeckhoven Christine Info: Researchers from VIB Center for Molecular Neurology, Antwerp Belgium and University of Antwerp assess … more »

Posted in Citation | Tagged , | Comments Off on Structural variants identified by Oxford Nanopore PromethION sequencing of the human genome

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the differentiation of embryonic stem cells towards pancreatic lineage and pancreatic beta cell function

May 2019 Authors: John A. Kubi, Andy C.H. Chen, Sze Wan Fong, Keng Po Lai, Chris K.C. Wong, William S.B. Yeung, Kai Fai Lee, Yin Lau Lee Info: From the University of Hong Kong and Hong Kong Baptist University, this … more »

Posted in Citation | Tagged , | Comments Off on Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the differentiation of embryonic stem cells towards pancreatic lineage and pancreatic beta cell function

Sequencing of human genomes with nanopore technology

April 2019 Authors: Rory Bowden, Robert W. Davies, Andreas Heger, Alistair T. Pagnamenta, Mariateresa de Cesare, Laura E. Oikkonen, Duncan Parkes, Colin Freema, Fatima Dhalla, Smita Y. Patel, Niko Popitsch, Camilla L.C. Ip, Hannah E. Roberts, Silvia Salatino, Helen Lockstone, … more »

Posted in Citation | Tagged , , , | Comments Off on Sequencing of human genomes with nanopore technology

Iso-Seq Allows Genome-Independent Transcriptome Profiling of Grape Berry Development

March 2019 Authors: Andrea Minio, Mélanie Massonnet, Rosa Figueroa-Balderas, Amanda M. Vondras, Barbara Blanco-Ulate, and Dario Cantu Info: Researchers used PacBio’s Iso-Seq method to sequence full-length cDNA transcripts from ripening Cabernet Savignon berries resulting in 1,5000 cultivar-specific genes. The authors … more »

Posted in Citation | Tagged , , | Comments Off on Iso-Seq Allows Genome-Independent Transcriptome Profiling of Grape Berry Development

Highly sensitive detection of mutations in CHO cell recombinant DNA using multi‐parallel single molecule real‐time DNA sequencing

June 2018 Authors: Joseph Cartwright, Karin Anderson, Joseph Longworth, Philip Lobb, and David James Info: To improve the quantitative assessment of recombinant DNA in cell lines, University of Sheffield scientists and collaborators developed a PacBio-based method for high-throughput rDNA sequence … more »

Posted in Citation | Tagged , | Comments Off on Highly sensitive detection of mutations in CHO cell recombinant DNA using multi‐parallel single molecule real‐time DNA sequencing

App Note: BluePippin Sizing Yields Longer MinION Reads

BluePippin users are generating longer reads from their Oxford Nanopore MinION sequencers, as demonstrated in a new app note from our colleagues at Nippon Genetics. The app note showcases a workflow used by Kazuharu Arakawa, an associate professor in the … more »

Posted in Blog | Tagged , , , | Comments Off on App Note: BluePippin Sizing Yields Longer MinION Reads

A Whole Genome Assembly of the Horn Fly, Haematobia irritans, and Prediction of Genes with Roles in Metabolism and Sex Determination

May 2018 Authors: Kranti Konganti, Felix Guerrero, Faye Schilkey, Peter Ngam, et al. Info: Researchers sequenced, assembled, and annotated the genome of horn fly, a blood-feeding insect that preys on cattle. The 1.14 Gb assembly shows the fly is most … more »

Posted in Citation | Tagged , | Comments Off on A Whole Genome Assembly of the Horn Fly, Haematobia irritans, and Prediction of Genes with Roles in Metabolism and Sex Determination

Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

April 2018 Authors: Paola Gaiero, Hana Šimková, JanVrána, Federico Santiñaque, et al. Info: Scientists from Wageningen University & Research and other institutions embarked on a project to improve sample prep protocols for obtaining high molecular weight DNA from plant cells … more »

Posted in Citation | Tagged , | Comments Off on Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

A Validation Approach of an End-to-End Whole Genome Sequencing Workflow for Source Tracking of Listeria monocytogenes and Salmonella enterica

March 2018 Authors: Anne-Catherine Portmann, Coralie Fournier, Johan Gimonet, Catherine Ngom-Bru, et al. Info: Scientists from Nestle Research Center and Nestle Institute of Health Sciences aimed to establish guidelines for whole-genome sequencing used in contamination investigations in the food industry. … more »

Posted in Citation | Tagged , | Comments Off on A Validation Approach of an End-to-End Whole Genome Sequencing Workflow for Source Tracking of Listeria monocytogenes and Salmonella enterica