Tag Archives: ChIP-Seq

Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome

March 2017 Authors: Warren Cheung, Xiaojian Shao, Andréanne Morin, Valérie Siroux, et al. Info: Researchers at McGill University and collaborating institutions used ChIP-seq to elucidate the effects of epigenetics and phenotype across the human genome. They analyzed more than 900 … more »

Posted in Citation | Tagged , , | Comments Off on Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome

Small genomic insertions form enhancers that misregulate oncogenes

February 2017 Authors: Brian J. Abraham, Denes Hnisz, Abraham S. Weintraub, Nicholas Kwiatkowski, et al. Info: In this paper, scientists report using ChIP-seq to better understand the role of non-coding somatic insertions in tumorigenesis. They identified enhancer-associated small insertions, often … more »

Posted in Citation | Tagged , , | Comments Off on Small genomic insertions form enhancers that misregulate oncogenes

Genome-wide identification of enhancer elements

June 2016 Authors: Sarah Tulin, Julius C. Barsi, Carlo Bocconcelli, and Joel Smith Info: The authors (from Woods Hole and Cal Tech) present a genome-wide regulatory element database for the sea urchin embryo. To do this, they developed a new … more »

Posted in Citation | Tagged , , , , | Comments Off on Genome-wide identification of enhancer elements

PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer

June 2016 Authors: Jennifer M. Spangle, Koen M. Dreijerink, Anna C. Groner, Hailing Cheng, Carolynn E. Ohlson, Jaime Reyes, Charles Y. Lin, James Bradner, Jean J. Zhao, Thomas M. Roberts, and Myles Brown Info: In this breast cancer research study, … more »

Posted in Citation | Tagged , , | Comments Off on PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer

Spotted in the Literature: The First PippinHT Publication!

We began shipping the high-throughput version of our automated Pippin DNA size selection platform last year, and it’s a thrill to see what we believe is the first reference to it in a peer-reviewed publication. A team of scientists from … more »

Posted in Blog | Tagged , , , , | Comments Off on Spotted in the Literature: The First PippinHT Publication!

PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab

At the Whitehead Institute’s Genome Technology Core, scientists handle a lot of ChIP-seq and RNA-seq projects. To boost capacity in library prep, they recently upgraded from a small fleet of Pippin Prep instruments to the new PippinHT for high-throughput, automated … more »

Posted in Blog | Tagged , , | Comments Off on PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab

Illumina Workflow: Pippin for ChIP-seq

As we continue our blog series on applications that are frequently used with Pippin size selection and Illumina sequencing, we move on to ChIP-seq. One of the most popular capabilities enabled by next-gen sequencing, ChIP-seq (or chromatin immunoprecipitation sequencing) is … more »

Posted in Blog | Tagged , , | Comments Off on Illumina Workflow: Pippin for ChIP-seq

As Sequencing Techs Mature, Scientists Look to Sample Prep for Improvements

We come to AGBT year after year because it is the place for new advances in DNA sequencing. But this year we’re hearing more and more about a cause that has special importance to us: sample prep. In the first … more »

Posted in Blog | Tagged , , , , | Comments Off on As Sequencing Techs Mature, Scientists Look to Sample Prep for Improvements

Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification

January 2014 Authors: Peter Tessarz, Helena Santos-Rosa, Sam C. Robson, Kathrine B. Sylvestersen, Christopher J. Nelson1, Michael L. Nielsen and Tony Kouzarides Info: The authors describe a new class of histone modification, methylation of glutamine, occurring on yeast histone H2A. … more »

Posted in Citation | Tagged , , , | Comments Off on Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification

Semiconductor-based DNA sequencing of histone modification states

October 2013 Authors: Christine S. Cheng, Kunal Rai, Manuel Garber, Andrew Hollinger, Dana Robbins, Scott Anderson, Alyssa Macbeth, Austin Tzou, Mauricio O. Carneiro, Raktima Raychowdhury, Carsten Russ, Nir Hacohen, Jeffrey E. Gershenwald, Niall Lennon, Chad Nusbaum, Lynda Chin, Aviv Regev … more »

Posted in Citation | Tagged , , | Comments Off on Semiconductor-based DNA sequencing of histone modification states