Tag Archives: Tips and Applications

What’s the difference between the 4-10 Kb vs2 and 6-10 Kb vs3 high pass protocols?

Customers may have noticed two very similar cassette definitions; 0.75%DF Marker S1 high-pass 4-10kb vs2 and 0.75%DF Marker S1 High-Pass 6-10kb vs3. Since these two protocols cover a similar range, they might seem a bit redundant. However, both of these … more »

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Pippin Pulse

Field Inversion Gel Electrophoresis       We’ve set up this post to provide Pippin Pulse users with a resource to keep updated on new protocols and to post references that might be useful. We also invite users to feel … more »

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And Now, a Word from Our Customer Service Department

Here at Sage Science, we are delighted to see more and more people signing up as customers of the Pippin platform. With so many instruments out in the wild, we thought it would be a good time to sit down … more »

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Band Capture with Midori Green on the BluePippin

Our band capture cassettes, for use on the BluePippin, are designed to automatically collect a band or fragment of DNA from a sample and remove unwanted DNA and/or primer artifacts. We’ve provided users with some flexibility to manipulate the capture … more »

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BluePippin “Mid-Range” Size Selection: 18-27 kb

We’re pleased to be shipping our Mid-Range size selection cassette (BMF7510) for the BluePippin. While our Low-Range cassette (BLF7510, 1kb-10kb) works for many mate-pair library construction protocols, we’ve been hearing from our customers that there is interest in creating larger … more »

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BluePippin:Collecting all DNA “Greater Than” 1kb

We have received several requests to use the Pippin to collect all remaining DNA above a programmed base pair value from a sample. Although Pippins currently have the capability to collect all DNA after a run time threshold (using the … more »

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New Cassettes Use Internal Standards

We’ve just released new dye-free cassettes for the BluePippin and Pippin Prep that use dye-labeled DNA markers* as internal standards, instead of typical external marker set. In the new product, the labeled markers will come premixed wi th the Ficoll … more »

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“Overflow Detection” definition on the Pippin Prep to be eliminated

| | Pippin Prep users are likely familiar with our “overflow detection” cassette definitions that are found in the “Cassette Type” drop down menu of the Protocol Editor. We use these definitions because Pippin cassettes exhibit a phenomenon where the … more »

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ChIP-Seq sample prep tip – Interpreting the apparent presence of a secondary band in size-selected DNA

ChIP-Seq analysis is used to study the control of gene expression by identifying DNA regions that are associated  with chromatin at the time of cellular analysis. The ChIP-Seq procedure typically involves cross-linking DNA to the associated chromatin proteins, sonication to … more »

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