Monthly Archives: October 2014

Evaluation of a Pyrenophora teres f. teres mapping population reveals multiple independent interactions with a region of barley chromosome 6H

August 2014 Authors: Rachel A. Shjerve, Justin D. Faris, Robert S. Brueggeman, Changhui Yan, Ya Zhu, Vaidehi Koladia, Timothy L. Friesen Info: Researchers in North Dakota studied the genetics of virulence in a fungal pathogen that affects barley. They looked … more »

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Genetic mapping of horizontal stripes in Lake Victoria cichlid fishes: benefits and pitfalls of using RAD markers for dense linkage mapping

August 2014 Authors: Frederico Henning, Hyuk Je Lee, Paolo Franchini and Axel Meyer Info: Scientists from the University of Konstanz in Germany use double-digest RADseq to determine the genetic basis for horizontal stripes seen on cichlid fishes. Sequencing was performed … more »

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Illumina Workflow: Pippin for Massively Parallel Genotyping

With so many Sage customers using their Pippin instruments in an Illumina sequencer pipeline, we’re taking a look at various applications enabled by the Sage + Illumina combination. Today we check out double-digest RADseq, which could not work without precise … more »

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Modified paired end rapid library preparation protocol for 454 GS Junior 8 kb library preparation using Covaris g-tubes and BluePippin electrophoresis

August 2014 Authors: Janet Hill, Bonnie Chaban, Jennifer Town, Matthew Links & Tim Dumonceaux Info: The scientists report a guide for 454 sample prep for paired-end reads. In their method, they eliminate the need for several expensive pieces of lab … more »

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