Monthly Archives: April 2015

DNA Day Makes Us Feel Like Rock Stars

This year’s DNA Day arrives at a heady time for advances with the world’s most important molecule: scientists have edited DNA in a human zygote for the first time, we’re closer to a fully finished human reference genome than ever … more »

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Assessing structural variation in a personal genome—towards a human reference diploid genome

April 2015 Authors: Adam C English, William J Salerno, Oliver A Hampton, Claudia Gonzaga-Jauregui, Shruthi Ambreth, Deborah I Ritter, Christine R Beck, Caleb F Davis, Mahmoud Dahdouli, Singer Ma, Andrew Carroll, Narayanan Veeraraghavan, Jeremy Bruestle, Becky Drees, Alex Hastie, Ernest … more »

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Improved transposon-based library preparation for the Ion Torrent platform

April 2015 Authors: Tatyana Gorbacheva, Wilber Quispe-Tintaya, Vasily N. Popov, Jan Vijg, and Alexander Y. Maslov Info: In this Biotechniques paper, scientists from the Albert Einstein College of Medicine in New York describe MuPlus, a new transposon-based method for barcoded … more »

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Video Protocol: NGS-based Methylation Mapping with Pippin Prep

We always love a great protocol video, and this one from scientists at Weill Cornell Medical College, published through the Journal of Visualized Experiments, is a keeper. Check it out here: “Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA … more »

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PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab

At the Whitehead Institute’s Genome Technology Core, scientists handle a lot of ChIP-seq and RNA-seq projects. To boost capacity in library prep, they recently upgraded from a small fleet of Pippin Prep instruments to the new PippinHT for high-throughput, automated … more »

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Discovery of Novel Rhabdoviruses in the Blood of Healthy Individuals from West Africa

March 2015 Authors: Matthew H Stremlau, Kristian G Andersen, Onikepe A Folarin, Jessica N Grove, Ikponmwonsa Odia, Philomena E Ehiane, Omowunmi Omoniwa, Omigie Omoregie, Pan-Pan Jiang, Nathan L Yozwiak, Christian B Matranga, Xiao Yang, Stephen K Gire, Sarah Winnicki, Ridhi … more »

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Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics

March 2015 Authors: Stephanie J Yaung, Luxue Deng, Ning Li, Jonathan L Braff, George M Church, Lynn Bry, Harris H Wang, Georg K Gerber Info: In this Molecular Systems Biology paper, researchers from Harvard and Columbia discuss the use of … more »

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TGAC Bioinformaticians Benefit from SageELF

Darren Heavens has witnessed a fascinating transition at The Genome Analysis Centre as the Norwich, UK-based institute shifted from data-generation mode to data-analysis mode. When the center launched more than five years ago, there was a fairly even split between … more »

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PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations

March 2015 Authors: Min Wang, Christine R Beck, Adam C English, Qingchang Meng, Christian Buhay, Yi Han, Harsha V Doddapaneni, Fuli Yu, Eric Boerwinkle, James R Lupski, Donna M Muzny and Richard A Gibbs Info: In this BMC Genomics publication, … more »

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New Structural Variant Analysis Method Uses Targeted Capture and Sequencing of Large-Insert DNA

In a new BMC Genomics paper, scientists from Baylor College of Medicine describe a new method for accurate, affordable interrogation of structural variants across the human genome. We’re delighted to see that automated DNA size selection tools from Sage Science … more »

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