Monthly Archives: July 2015

Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

July 2015 Authors: Juan Pablo Lopez, Alpha Diallo, Cristiana Cruceanu, Laura M. Fiori, Sylvie Laboissiere, Isabelle Guillet, Joelle Fontaine, Jiannis Ragoussis, Vladimir Benes, Gustavo Turecki, and Carl Ernst Info: Scientists from McGill University and the European Molecular Biology Laboratory evaluated … more »

Posted in Citation | Tagged , | Comments Off on Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo

July 2015 Authors: Yi-Zhou Jiang, Elisabetta Manduchi, Christian J. Stoeckert, and Peter F. Davies Info: In this paper, scientists from the University of Pennsylvania used complete methylated DNA immunoprecipitation sequencing (MeDIP-seq) to study methylation differences in endothelial cells to find … more »

Posted in Citation | Tagged , | Comments Off on Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo

A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost

July 2015 Authors: Darren Heavens, Gonzalo Garcia Accinelli, Bernardo Clavijo, and Matthew Derek Clark Info: Scientists from The Genome Analysis Centre report a protocol using the SageELF to improve library preparation for long mate-pair sequencing. Using a Nextera kit, they … more »

Posted in Citation | Tagged , , | Comments Off on A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost

Scientists Optimize Mate-Pair Sequencing with SageELF

In a Biotechniques paper this month, scientists from The Genome Analysis Centre describe a new method for mate-pair sequencing that saves time and money while decreasing the amount of input DNA required. The method is based on SageELF, which automatically … more »

Posted in Blog | Tagged , , , | Comments Off on Scientists Optimize Mate-Pair Sequencing with SageELF

Towards allele-level human leucocyte antigens genotyping – assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq

June 2015 Authors: J. L. Duke, C. Lind, K. Mackiewicz, D. Ferriola, A. Papazoglou, O. Derbeneva, D. Wallace, and D. S. Monos Info: This publication in the International Journal of Immunogenetics from scientists at the Children’s Hospital of Philadelphia presents … more »

Posted in Citation | Tagged , , , | Comments Off on Towards allele-level human leucocyte antigens genotyping – assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq

Assembly and diploid architecture of an individual human genome via single-molecule technologies

June 2015 Authors: Matthew Pendleton, Robert Sebra, Andy Wing Chun Pang, Ajay Ummat, Oscar Franzen, Tobias Rausch, Adrian M Stütz, William Stedman, Thomas Anantharaman, Alex Hastie, Heng Dai, Markus Hsi-Yang Fritz, Han Cao, Ariella Cohain, Gintaras Deikus, Russell E Durrett, … more »

Posted in Citation | Tagged , | Comments Off on Assembly and diploid architecture of an individual human genome via single-molecule technologies

Hybrid Single-Molecule Approach Produces Highly Contiguous Diploid Human Genome Assembly

A team of scientists from the Icahn School of Medicine at Mount Sinai, Weill Cornell Medical College, Cold Spring Harbor Laboratory, European Molecular Biology Laboratory, and other institutions published the first analysis of a diploid human genome produced by combining … more »

Posted in Blog | Tagged , | Comments Off on Hybrid Single-Molecule Approach Produces Highly Contiguous Diploid Human Genome Assembly