Churning Out New Microbial Genome Sequences

PacBio users have been regularly serving up new microbial genome assemblies, and we’re glad to see that they’re using our BluePippin automated DNA size selection instrument to get the best results.

These are just some of the genome announcements published in the last few months:

Erwinia tracheiphila
A pathogen affecting economically important crops, such as melons and gourds, which had not previously been sequenced. Scientists present a draft sequence containing seven contigs and many phage or prophage elements.

Clostridium sporogenes DSM 795T
Researchers published this first whole genome sequence of this bacterium, a nontoxigenic relative of Clostridium botulinum. The genome was finished into a single contig of about 4 Mb and contains dozens of identical sequence copies greater than 1,000 bases.

Sedimenticola thiotaurini
A member of a group of sulfur-oxidizing bacteria, Sedimenticola thiotaurini strain SIP-G1 was sequenced and presented as a closed genome assembly. Scientists identified pathways not found in other members of this genus.

Microcystis aeruginosa
Scientists sequenced and annotated Microcystis aeruginosa NIES-2549, a freshwater cyanobacterium. The genome is almost 4.3 Mb and was sequenced to help understand the species’ ability to produce hepatotoxic cyanotoxins, which cause major environmental damage.

Escherichia coli O96:H19
This E. coli strain was responsible for a foodborne outbreak in Milan last year in which the organism’s pathogenicity was far more severe than usual. The published genome sequence is fully closed and allows scientists to study its acquired virulence.

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