Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization

December 2016

Authors:
Peter M. Clark, Jamie L. Duke, Deborah Ferriola, Valia Bravo-Egana, Tunde Vago, Aniqa Hassan, Anna Papazoglou, and Dimitri Monos

Info:
Scientists from The Children’s Hospital of Philadelphia evaluated an NGS-based HLA typing kit from Omixon. They analyzed 50 samples; 7.7% were found to have novel polymorphisms, suggesting that new sequencing approaches could usher in a new era of discovery for HLA alleles. The team used Pippin Prep to size-select libraries prior to sequencing on a MiSeq.

Citation:
Clinical Chemistry
DOI: 10.1373/clinchem.2016.260661

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