Citations

Ingestible Osmotic Pill for In‐vivo Sampling of Gut Microbiome

July 2019

Authors:
Hojatollah Rezaei Nejad, Bruno C. M. Oliveira, Aydin Sadeqi, Amin Dehkharghani, Ivanela Kondova Jan A. M. Langermans, Jeffrey S. Guasto, Saul Tzipori, Giovanni Widmer, Sameer R. Sonkusale

Info:
From Engineering and Nano Labs at Tufts University, and a consortium of Veterinary groups from the Netherlands, Brazil, and Tufts. A 3D printed micro-engineered pill with osmotic pumping technology was developed to sample the gut microbiome in animals. The pill was coated to resist exposure to the stomach microbiome and can be magnetically held in the upper intestinal track until sampling is complete.

The system was validated in vitro and in vivo. The Pippin Prep was used to size select 16S amplicons prior to Illumina sequencing.

Citation:
Advanced Intelligent Systems
DOI: 10.1002/aisy.201900053

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Structural variants identified by Oxford Nanopore PromethION sequencing of the human genome

June 2019

Authors:
De Coster Wouter, De Rijk Peter, De Roeck Arne, De Pooter Tim, D’Hert Svenn, Strazisar Mojca, Kristel Sleegers and Van Broeckhoven Christine

Info:
Researchers from VIB Center for Molecular Neurology, Antwerp Belgium and University of Antwerp assess using the ONT Promethion for Structural Varian detection in the genome of Yoruban reference individual NA19240. Several variant calling tools were evaluated and compared to short-read sequencing variant calls. A scalable workflow is discussed and future study is considered.
The BluePippin was used for High Pass DNA size selection prior to library construction.

Citation:
Genome Research
DOI: 10.1101/gr.244939.118

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Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the differentiation of embryonic stem cells towards pancreatic lineage and pancreatic beta cell function

May 2019

Authors:
John A. Kubi, Andy C.H. Chen, Sze Wan Fong, Keng Po Lai, Chris K.C. Wong,
William S.B. Yeung, Kai Fai Lee, Yin Lau Lee

Info:
From the University of Hong Kong and Hong Kong Baptist University, this study examines the effect of low exposure of a dioxin (TCDD) on embryonic stem cells. Previous animal studies had linked high dioxin exposure to susceptibility to type 2 diabetes. Results indicated altered methylation patterns in stem cell genes associated with insulin secretion. The authors suggest that early embryonic exposure to TCDD may affect pancreatogenesis.
The BluePippin was used to size select libraries prior to bisulfite conversion and reduced representation bisulfite sequencing.

Citation:
Environment International
DOI: 10.1016/j.envint.2019.05.079

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Systematic sequencing of chloroplast transcript termini from Arabidopsis thaliana reveals >200 transcription initiation sites and the extensive imprints of RNA-binding proteins and secondary structures

April 2019

Authors:
Benoît Castandet, Arnaud Germain, Amber M. Hotto, and David B. Stern
Info:
Researchers present a protocol called Terminome-Seq that they developed to catalog RNA transcript termini in chloroplast DNA of Arabidopsis thaliana. RNA-seq libraries are prepared for the simultaneous analysis of primary and processed 5’ ends, and 3’ends. The authors indicate that the method helps define promoter sequences, regulatory untranslated regions, and the potential for sense-antisense pairing.

Pippin Prep was used to remove products below 200 bp.

Author Affiliations:
Boyce Thompson Institute, Ithica NY
Cornell University, Ithaca,
Université Paris-Saclay, France

Citation:
BioRxiv
DOI: 10.1101/621938

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Museum epigenomics: characterizing cytosine methylation in historic museum specimens

April 2019

Authors:
Tricia L. Rubi, L. Lacey Knowles, Ben Dantzer

Info:
In this preprint, researchers developed method the study epigenetic markers on historically preserved deer mouse specimens using ddRAD-seq and bisulfite sequencing. The oldest group of specimens was 76 years old.

The Pippin Prep was used to size select ddRAD libraries prepared with an extra bisulfite conversion step. Size selection: 376-412 bp and 325-425 bp for higher and lower concentration preps, respectively.

Author Affiliations:
University of Michigan

Citation:
BioRxiv
DOI: 10.1101/620583

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