Genomic architecture of ecologically divergent body shape in a pair of sympatric crater lake cichlid fishes
October 2013
Authors:
PAOLO FRANCHINI, CARMELO FRUCIANO, MARIA L. SPREITZER, JULIA C. JONES, KATHRYN R. ELMER, FREDERICO HENNING and AXEL MEYER
Info:
Researchers at the University of Konstanz, Germany, look at the speciation of chichlid fishes using the ddRAD technique. The Pippin Prep was used to size-select prior to Illumina sequencing. The study indicates a few genomic regions that largely contributed to early divergence.
Citation:
Molecular Ecology (2013)
Improved Protocols for Illumina Sequencing
October 2013
Authors:
Iraad F. Bronner, Michael A. Quail, Daniel J. Turner, and Harold Swerdlow
Info:
A comprehensive overview of the state-of-the state of Illumina sequencing, from the Wellcome Trust Sanger Institute and Oxford Nanopore Technologies. The value of narrow size distribution and Pippin automated size selection is discussed.
Citation:
Current Protocols in Human Genetics 18.2.1-18.2.42, October 2013
Next-Generation Sequence Analysis of Cancer Xenograft Models
Authors:
Fernando J. Rossello, Richard W. Tothill, Kara Britt, Kieren D. Marini, Jeanette Falzon, David M. Thomas, Craig D. Peacock, Luigi Marchionni, Jason Li, Samara Bennett, Erwin Tantoso, Tracey Brown, Philip Chan, Luciano G. Martelotto, D. Neil Watkins
Info:
The authors looked at using xenografts as preclinical models for NGS cancer studies. The authors were able to show that mouse specific sequence was easily distinguished from human small cell lung cancer sequences. Pippin Prep size selection was used for whole genome library construction for the Illumina HiSeq.
Citation:
PLoS ONE 8(9): e74432
Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway
Authors:
Tao-Yi Cai, Wei Zhu, Xiong-Sheng Chen, Sheng-Yuan Zhou, Lian-Shun Jia and Yan-Qing Sun
Info:
From the Second Military Medical University in Shanghai, China, this qPCR gene expression study looks at the effect of fibroblast growth factor mesenchymal stems cells. The BluePippin was used to size-select cDNAs.
Citation:
Molecular Medicine Reports 8: 1323-1328, 2013
De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana
August 2013
Authors:
Stephen M Gross, Jeffrey A Martin, June Simpson, María Jazmín Abraham-Juarez, Zhong Wang and Axel Visel
Info:
Agave is an interesting plant for bioenergy study. This paper provides transcriptome data of two strains. The Pippin Prep was used to size select cDNAs at 250 or 500 bp, followed by TrueSeq library construction and sequencing on the Illumina HiSeq.
Citation:
BMC Genomics 2013 14:563