Tag Archives: Mate-pair Sequencing

Mate-Pair Sequencing Is Having a Moment

Have you been noticing the mate-pair trend? We certainly have. A technique that was once only used by a handful of labs has really come into its own as one of the preferred ways of gathering longer-range genomic data. Here … more »

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New Resources: App Notes for Mate-Pair and Long-Read Sequencing with BluePippin

We’ve got some new application notes to share that will be particularly handy for BluePippin customers running mate-pair libraries or sequencing with the Pacific Biosciences platform. Many thanks to our distribution partner, Nippon Genetics, for making this great information available … more »

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Illumina Workflow: Pippin or SageELF for Mate-Pair Sequencing

Most Sage customers use their Pippins with an Illumina workflow, so for the next few weeks we’ll be taking a look at some of the most common or interesting applications of automated DNA sizing with the HiSeq, MiSeq, and even … more »

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Discovery of transgene insertion sites by high throughput sequencing of mate pair libraries

May 2014 Authors: Anuj Srivastava, Vivek M Philip, Ian Greenstein, Lucy B Rowe, Mary Barter, Cathleen Lutz and Laura G Reinholdt Info: Jackson Laboratory scientists developed a method to characterize and score transgene insertion sites in mice to help researchers … more »

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In Sanger Study, BluePippin Contributes to Improved Construction Method for Mate-Pair Libraries

Scientists from the Wellcome Trust Sanger Institute recently published “An improved approach to mate-paired library preparation for Illumina sequencing,” a paper that came out in the open access journal Methods in Next Generation Sequencing. The publication, from lead author Naomi … more »

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At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies

Before a new technology is deployed at The Genome Analysis Centre in Norwich, UK, it must run the gauntlet of Matthew Clark’s lab. Clark is the sequencing technology development leader at TGAC, where part of his role involves testing out … more »

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Pippin Platform Recommended for Nextera Mate Pair Size Selection

Illumina released new sample prep protocol guidelines for generating mate pair libraries with its Nextera kit, and we’re pleased to report that the Pippin platform is the recommended choice for automated size selection. You can check out the Nextera Mate … more »

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For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies

At the DNA Technologies Laboratory at the National Research Council of Canada, scientists are using the Pippin size selection platform to improve the quality of their genome assemblies. Andrew Sharpe, Research Officer and Group Leader of the Saskatoon-based laboratory, got … more »

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At Emory, Size Selection Saves Time for NGS Prep

At the genome center at Emory University, scientists credit the Pippin Prep with shaving almost a full day off the sample prep process for Illumina’s mate-pair library prep. Chad Haase, laboratory manager at the Emory GRA Genome Center, says that … more »

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