Tag Archives: Paired-end Sequencing

A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology

June 2013 Authors: A. Murat Eren, Joseph H. Vineis., Hilary G. Morrison, Mitchell L. Sogin Info: The authors present a method of library preparation for overlapping paired-end sequencing on the Illumina platform, and improved analysis software, to reduce the error … more »

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Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene

January 2013 Authors: Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins Info: The authors designed a general method to study the arrangement of foreign DNA and host … more »

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Bactobolin Resistance is Conferred by Mutations in the L2 Ribosomal Protein

December 2012 Authors: Josephine Chandler, Thao T. Truong, Patricia M. Silva, Mohammad R. Seyedsayamdost, Galvin Carr, Matthew Radey, Michael A. Jacobs, Elizabeth H. Sims, Jon Clardy, E. Peter Greenberg Info: Authors were searching for cause of resistance to antibiotic Bactobolin. … more »

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The Use of NexGen Sequencing and Junction Sequence Analysis Bioinformatics to Achieve Molecular Characterization of Crops Improved Through Modern Biotechnology

October 2012 Authors: David Kovalic, Carl Garnaat, Liang Guo, Yongpan Yan, Jeanna Groat, Andre Silvanovich, Lyle Ralston, Mingya Huang, Qing Tian, Allen Christian, Nordine Cheikh, Jerry Hjelle, Stephen Padgette and Gary Bannon Info: Materials and Methods: Illumina TruSeq paired-end library … more »

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Mapping the Hallmarks of Lung Adenocarcinoma with Massively Parallel Sequencing

September 2012 Authors: Marcin Imielinski et al. Info: A landmark study of lung cancer genomes with 183 tumor/normal genomes compared. Sequencing was carried out mainly by the Broad Illumina Platform (8 Pippins in production). The Pippin Prep is cited in … more »

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For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies

At the DNA Technologies Laboratory at the National Research Council of Canada, scientists are using the Pippin size selection platform to improve the quality of their genome assemblies. Andrew Sharpe, Research Officer and Group Leader of the Saskatoon-based laboratory, got … more »

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Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression

March 2012 Authors: Linzhao Cheng, Nancy F. Hansen, Ling Zhao, Yutao Du, Chunlin Zou, Frank X. Donovan, Bin-Kuan Chou, Guangyu Zhou, Shijie Li, Sarah N. Dowey, Zhaohui Ye, NISC Comparative Sequencing Program, Settara C. Chandrasekharappa, Huanming Yang, James C. Mullikin, … more »

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Mutation discovery in mice by whole exome sequencing

December 2011 Authors: Heather Fairfield, Griffith J Gilbert, Mary Barter, Rebecca R Corrigan, Michelle Curtain, Yueming Ding, Mark D’Ascenzo, Daniel J Gerhardt, Chao He, Wenhui Huang, Todd Richmond, Lucy Rowe, Frank J Probst, David E Bergstrom, Stephen A Murray, Carol … more »

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Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn

September 2011 Authors: Laura G. Reinholdt, Yueming Ding, Griffith T. Gilbert, Anne Czechanski, Jeffrey P. Solzak, Randall J. Roper, Mark T. Johnson, Leah Rae Donahue, Cathleen Lutz, Muriel T. Davisson Info: Illumina PE libraries were constructed and size selected at … more »

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