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Tag Archives: PippinHT
ATAC-seq with unique molecular identifiers improves quantification and footprinting
November 2020 Authors: Tao Zhu, Keyan Liao, Rongfang Zhou, Chunjiao Xia & Weibo Xie Info: The authors suggest an improvement on the ATAC-seq method (Assay for Transposase-Accessible Chromatin with high-throughput sequencing). By using unique molecular identifiers to distinguish between transposase … more »
Distinct Profiles of Cell-Free MicroRNAs in Plasma of Veterans with Post-Traumatic Stress Disorder
July 2019 Authors: Min Young Lee, David Baxter, Kelsey Scherler , Taek-Kyun Kim, Xiaogang Wu , Duna Abu-Amara, Janine Flory, Rachel Yehuda, Charles Marma, Marti Jett, Inyoul Lee, Kai Wang, and Leroy Hood Info: Researchers report on studies that profile … more »
Comparison of RNA isolation and library preparation methods for small RNA sequencing of canine biofluids
Authors: Candice P. Chu, Mary B. Nabity Info: From the Department of Veterinary Pathobiology at Texas A&M, this study evaluates best practices for small RNA sequencing from canine biofluid samples (serum and urine). The study compares RNA-seq results after using … more »
Identification of whole blood mRNA and microRNA biomarkers of tissue damage and immune function resulting from amphetamine exposure or heat stroke in adult male rats
February 2019 Authors: Luısa Camacho, Camila S. Silva, Joseph P. Hanig, Robert P. Schleimer, Nysia I. George, John F. Bowyer Info: A report from the USDA and others evaluate the effect of amphetamine and methamphetamine toxicity on immune systems and … more »
Cancer stem cell–associated miRNAs serve as prognostic biomarkers in colorectal cancer
March 2019 Authors: Shusuke Toden, Shigeyasu Kunitoshi, Jacob Cardenas, Jinghua Gu, Elizabeth Hutchins, Kendall Van Keuren-Jensen, Hiroyuki Uetake, Yuji Toiyama, and Ajay Goel Info: This study evaluates cancer stems cells (CSC) which resist chemotherapy agents and have high tumor initiation … more »
Gestational jet lag predisposes to later-life skeletal and cardiac disease
February 2019 Authors: Inês Chaves, Bram van der Eerden, Ruben Boers, Joachim Boers, Astrid A. Streng, Yanto Ridwan, Marijke Schreuders-Koedam, Marijn Vermeulen, Ingrid van der Pluijm, Jeroen Essers, Joost Gribnau, Irwin K. M. Reiss & Gijsbertus T. J. van der … more »
Posted in Citation
Tagged CpG island, MED-seq, methylation, PippinHT
Comments Off on Gestational jet lag predisposes to later-life skeletal and cardiac disease
Assembly and annotation of a draft genome sequence for Glycine latifolia, a perennial wild relative of soybean
April 2018 Authors: Qiong Liu, Sungyul Chang, Glen Hartman, and Leslie Domier Info: At the University of Illinois, scientists sequenced a wild perennial relative of the soybean plant, producing a 939 Mb draft assembly using linked reads from a single … more »
Posted in Citation
Tagged 10x Genomics, PippinHT
Comments Off on Assembly and annotation of a draft genome sequence for Glycine latifolia, a perennial wild relative of soybean
Transmission of microRNA antimiRs to mouse offspring via the maternal-placental-fetal unit
March 2018 Authors: Jonas Hönig, Ivana Mižíková, Claudio Nardiello, David Emanuel Surate Solaligue, et al. Info: In Germany, researchers delivered microRNA inhibitors to pregnant mice, finding that the antimiRs were then detectable in offspring, where they caused fewer off-target effects … more »
New Protocol: Analyze Small Extracellular RNAs with PippinHT
Good protocols are the currency of any lab. If your research involves working with small RNAs, be sure to check out this carefully documented protocol published in Extracellular RNA, part of Springer Nature’s Methods in Molecular Biology book series. The protocol … more »
Improving saliva shotgun metagenomics by chemical host DNA depletion
February 2018 Authors: Clarisse Marotz, Jon Sanders, Cristal Zuniga, Livia Zaramela, Rob Knight, and Karsten Zengler Info: Scientists from the University of California, San Diego, developed and evaluated a new protocol for enriching microbial DNA collected from fresh and frozen … more »