Tag Archives: Pippin Pulse

Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

April 2018 Authors: Paola Gaiero, Hana Šimková, JanVrána, Federico Santiñaque, et al. Info: Scientists from Wageningen University & Research and other institutions embarked on a project to improve sample prep protocols for obtaining high molecular weight DNA from plant cells … more »

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Assembly and RNA‐free annotation of highly heterozygous genomes: The case of the thick‐billed murre (Uria lomvia)

January 2018 Authors: Anna Tigano, Timothy Sackton, and Vicki Friesen Info: Researchers at Queen’s University and Harvard teamed up for an effort to assemble the highly heterozygous genome of an arctic colonial seabird. The found that the platanus assembler outperformed … more »

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Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method

September 2017 Authors: Kengo Kato, Masanori Hashino, Tamaki Ito, Mari Matsui, et al. Info: Researchers developed and validated a streamlined protocol for PacBio sequencing sample prep. The method involves bead-beating for DNA isolation. For the project, the team used Pippin … more »

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Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing

June 2016 Authors: Shruthi Sridhar Vembar, Matthew Seetin, Christine Lambert, Maria Nattestad, Michael C. Schatz, Primo Baybayan, Artur Scherf, and Melissa Laird Smith Info: Scientists in France and the US used PacBio sequencing to characterize the AT-rich genome of this … more »

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